Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability.
Identifieur interne : 000025 ( Main/Exploration ); précédent : 000024; suivant : 000026Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability.
Auteurs : Karla Ili Ur I [Serbie] ; Selin Ece [Allemagne] ; Raluca Ostafe [Allemagne] ; Simon Vogel [Allemagne] ; Ana Marija Balaž [Serbie] ; Stefan Schillberg [Allemagne] ; Rainer Fischer [États-Unis] ; Radivoje Prodanovi [Serbie]Source :
- Journal of bioscience and bioengineering [ 1347-4421 ] ; 2020.
Descripteurs français
- KwdFr :
- Banque de gènes (MeSH), Cytométrie en flux (MeSH), Oxydoréduction (MeSH), Peroxidases (génétique), Peroxidases (métabolisme), Peroxyde d'hydrogène (métabolisme), Phanerochaete (enzymologie), Phanerochaete (génétique), Saccharomyces cerevisiae (génétique), Saccharomyces cerevisiae (métabolisme), Stress oxydatif (MeSH).
- MESH :
- enzymologie : Phanerochaete.
- génétique : Peroxidases, Phanerochaete, Saccharomyces cerevisiae.
- métabolisme : Peroxidases, Peroxyde d'hydrogène, Saccharomyces cerevisiae.
- Banque de gènes, Cytométrie en flux, Oxydoréduction, Stress oxydatif.
English descriptors
- KwdEn :
- Flow Cytometry (MeSH), Gene Library (MeSH), Hydrogen Peroxide (metabolism), Oxidation-Reduction (MeSH), Oxidative Stress (MeSH), Peroxidases (genetics), Peroxidases (metabolism), Phanerochaete (enzymology), Phanerochaete (genetics), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (metabolism).
- MESH :
- chemical , genetics : Peroxidases.
- chemical , metabolism : Hydrogen Peroxide, Peroxidases.
- enzymology : Phanerochaete.
- genetics : Phanerochaete, Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- Flow Cytometry, Gene Library, Oxidation-Reduction, Oxidative Stress.
Abstract
Lignin peroxidase (LiP) is a heme-containing oxidoreductase that oxidizes structurally diverse substrates in an H2O2-dependent manner. Its ability to oxidize many pollutants makes it suitable for bioremediation applications and an ideal candidate for optimization by mutagenesis and selection. In order to increase oxidative stability of LiP we generated a random mutagenesis library comprising 106 mutated LiP genes and screened for expressed enzymes with higher than wild-type activity after incubation in 30 mM H2O2 by flow cytometry with fluorescein-tyramide as a substrate. To preserve the genotype-phenotype connection, the LiP mutants were displayed on the yeast cell surface. Two rounds of sorting were performed, recovered colonies were then screened in microtiter plates, and activity analysis revealed a significant increase in the percentage of cells expressing LiP variants with higher oxidative stability than wtLiP. Two rounds of sorting increased the proportion of more-stable variants from 1.4% in the original library to 52.3%. The most stable variants after two rounds of sorting featured between two and four mutations and retained up to 80% of initial activity after 1 h incubation in 30 mM H2O2. We for the first-time applied flow cytometry for screening of any ligninolytic peroxidase library. Obtained results suggest that developed system may be applied for improvement of industrially important characteristics of lignin peroxidase.
DOI: 10.1016/j.jbiosc.2019.12.009
PubMed: 32035791
Affiliations:
- Allemagne, Serbie, États-Unis
- District de Cologne, Indiana, Rhénanie-du-Nord-Westphalie
- Aix-la-Chapelle
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability.</title><author><name sortKey="Ili Ur I, Karla" sort="Ili Ur I, Karla" uniqKey="Ili Ur I K" first="Karla" last="Ili Ur I">Karla Ili Ur I</name><affiliation wicri:level="1"><nlm:affiliation>University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade, Serbia. Electronic address: karlailic@chem.bg.ac.rs.</nlm:affiliation><country xml:lang="fr">Serbie</country><wicri:regionArea>University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade</wicri:regionArea><wicri:noRegion>11000 Belgrade</wicri:noRegion></affiliation></author><author><name sortKey="Ece, Selin" sort="Ece, Selin" uniqKey="Ece S" first="Selin" last="Ece">Selin Ece</name><affiliation wicri:level="1"><nlm:affiliation>PerkinElmer Chemagen Technologie GmbH, Arnold-Sommerfeld-Ring 2, 52499 Baesweiler, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>PerkinElmer Chemagen Technologie GmbH, Arnold-Sommerfeld-Ring 2, 52499 Baesweiler</wicri:regionArea><wicri:noRegion>52499 Baesweiler</wicri:noRegion><wicri:noRegion>52499 Baesweiler</wicri:noRegion><wicri:noRegion>52499 Baesweiler</wicri:noRegion></affiliation></author><author><name sortKey="Ostafe, Raluca" sort="Ostafe, Raluca" uniqKey="Ostafe R" first="Raluca" last="Ostafe">Raluca Ostafe</name><affiliation wicri:level="3"><nlm:affiliation>Purdue Institute of Inflammation, Immunology and Infectious Disease, Molecular Evolution, Protein Engineering and Production, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907, USA; Institute of Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Purdue Institute of Inflammation, Immunology and Infectious Disease, Molecular Evolution, Protein Engineering and Production, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907, USA; Institute of Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation></author><author><name sortKey="Vogel, Simon" sort="Vogel, Simon" uniqKey="Vogel S" first="Simon" last="Vogel">Simon Vogel</name><affiliation wicri:level="3"><nlm:affiliation>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation></author><author><name sortKey="Balaz, Ana Marija" sort="Balaz, Ana Marija" uniqKey="Balaz A" first="Ana Marija" last="Balaž">Ana Marija Balaž</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chemistry Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000 Belgrade, Serbia.</nlm:affiliation><country xml:lang="fr">Serbie</country><wicri:regionArea>Institute of Chemistry Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000 Belgrade</wicri:regionArea><wicri:noRegion>11000 Belgrade</wicri:noRegion></affiliation></author><author><name sortKey="Schillberg, Stefan" sort="Schillberg, Stefan" uniqKey="Schillberg S" first="Stefan" last="Schillberg">Stefan Schillberg</name><affiliation wicri:level="3"><nlm:affiliation>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation></author><author><name sortKey="Fischer, Rainer" sort="Fischer, Rainer" uniqKey="Fischer R" first="Rainer" last="Fischer">Rainer Fischer</name><affiliation wicri:level="2"><nlm:affiliation>Institute of Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany; Departments of Biological Sciences and Chemistry, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute of Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany; Departments of Biological Sciences and Chemistry, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907</wicri:regionArea><placeName><region type="state">Indiana</region></placeName></affiliation></author><author><name sortKey="Prodanovi, Radivoje" sort="Prodanovi, Radivoje" uniqKey="Prodanovi R" first="Radivoje" last="Prodanovi">Radivoje Prodanovi</name><affiliation wicri:level="1"><nlm:affiliation>University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade, Serbia.</nlm:affiliation><country xml:lang="fr">Serbie</country><wicri:regionArea>University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade</wicri:regionArea><wicri:noRegion>11000 Belgrade</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32035791</idno><idno type="pmid">32035791</idno><idno type="doi">10.1016/j.jbiosc.2019.12.009</idno><idno type="wicri:Area/Main/Corpus">000026</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000026</idno><idno type="wicri:Area/Main/Curation">000026</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000026</idno><idno type="wicri:Area/Main/Exploration">000026</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability.</title><author><name sortKey="Ili Ur I, Karla" sort="Ili Ur I, Karla" uniqKey="Ili Ur I K" first="Karla" last="Ili Ur I">Karla Ili Ur I</name><affiliation wicri:level="1"><nlm:affiliation>University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade, Serbia. Electronic address: karlailic@chem.bg.ac.rs.</nlm:affiliation><country xml:lang="fr">Serbie</country><wicri:regionArea>University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade</wicri:regionArea><wicri:noRegion>11000 Belgrade</wicri:noRegion></affiliation></author><author><name sortKey="Ece, Selin" sort="Ece, Selin" uniqKey="Ece S" first="Selin" last="Ece">Selin Ece</name><affiliation wicri:level="1"><nlm:affiliation>PerkinElmer Chemagen Technologie GmbH, Arnold-Sommerfeld-Ring 2, 52499 Baesweiler, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>PerkinElmer Chemagen Technologie GmbH, Arnold-Sommerfeld-Ring 2, 52499 Baesweiler</wicri:regionArea><wicri:noRegion>52499 Baesweiler</wicri:noRegion><wicri:noRegion>52499 Baesweiler</wicri:noRegion><wicri:noRegion>52499 Baesweiler</wicri:noRegion></affiliation></author><author><name sortKey="Ostafe, Raluca" sort="Ostafe, Raluca" uniqKey="Ostafe R" first="Raluca" last="Ostafe">Raluca Ostafe</name><affiliation wicri:level="3"><nlm:affiliation>Purdue Institute of Inflammation, Immunology and Infectious Disease, Molecular Evolution, Protein Engineering and Production, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907, USA; Institute of Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Purdue Institute of Inflammation, Immunology and Infectious Disease, Molecular Evolution, Protein Engineering and Production, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907, USA; Institute of Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation></author><author><name sortKey="Vogel, Simon" sort="Vogel, Simon" uniqKey="Vogel S" first="Simon" last="Vogel">Simon Vogel</name><affiliation wicri:level="3"><nlm:affiliation>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation></author><author><name sortKey="Balaz, Ana Marija" sort="Balaz, Ana Marija" uniqKey="Balaz A" first="Ana Marija" last="Balaž">Ana Marija Balaž</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chemistry Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000 Belgrade, Serbia.</nlm:affiliation><country xml:lang="fr">Serbie</country><wicri:regionArea>Institute of Chemistry Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000 Belgrade</wicri:regionArea><wicri:noRegion>11000 Belgrade</wicri:noRegion></affiliation></author><author><name sortKey="Schillberg, Stefan" sort="Schillberg, Stefan" uniqKey="Schillberg S" first="Stefan" last="Schillberg">Stefan Schillberg</name><affiliation wicri:level="3"><nlm:affiliation>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation></author><author><name sortKey="Fischer, Rainer" sort="Fischer, Rainer" uniqKey="Fischer R" first="Rainer" last="Fischer">Rainer Fischer</name><affiliation wicri:level="2"><nlm:affiliation>Institute of Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany; Departments of Biological Sciences and Chemistry, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute of Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany; Departments of Biological Sciences and Chemistry, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907</wicri:regionArea><placeName><region type="state">Indiana</region></placeName></affiliation></author><author><name sortKey="Prodanovi, Radivoje" sort="Prodanovi, Radivoje" uniqKey="Prodanovi R" first="Radivoje" last="Prodanovi">Radivoje Prodanovi</name><affiliation wicri:level="1"><nlm:affiliation>University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade, Serbia.</nlm:affiliation><country xml:lang="fr">Serbie</country><wicri:regionArea>University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade</wicri:regionArea><wicri:noRegion>11000 Belgrade</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of bioscience and bioengineering</title><idno type="eISSN">1347-4421</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Flow Cytometry (MeSH)</term><term>Gene Library (MeSH)</term><term>Hydrogen Peroxide (metabolism)</term><term>Oxidation-Reduction (MeSH)</term><term>Oxidative Stress (MeSH)</term><term>Peroxidases (genetics)</term><term>Peroxidases (metabolism)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (genetics)</term><term>Saccharomyces cerevisiae (genetics)</term><term>Saccharomyces cerevisiae (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Banque de gènes (MeSH)</term><term>Cytométrie en flux (MeSH)</term><term>Oxydoréduction (MeSH)</term><term>Peroxidases (génétique)</term><term>Peroxidases (métabolisme)</term><term>Peroxyde d'hydrogène (métabolisme)</term><term>Phanerochaete (enzymologie)</term><term>Phanerochaete (génétique)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Saccharomyces cerevisiae (métabolisme)</term><term>Stress oxydatif (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peroxidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hydrogen Peroxide</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Peroxidases</term><term>Phanerochaete</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Peroxidases</term><term>Peroxyde d'hydrogène</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Flow Cytometry</term><term>Gene Library</term><term>Oxidation-Reduction</term><term>Oxidative Stress</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Banque de gènes</term><term>Cytométrie en flux</term><term>Oxydoréduction</term><term>Stress oxydatif</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lignin peroxidase (LiP) is a heme-containing oxidoreductase that oxidizes structurally diverse substrates in an H<sub>2</sub>O<sub>2</sub>-dependent manner. Its ability to oxidize many pollutants makes it suitable for bioremediation applications and an ideal candidate for optimization by mutagenesis and selection. In order to increase oxidative stability of LiP we generated a random mutagenesis library comprising 10<sup>6</sup> mutated LiP genes and screened for expressed enzymes with higher than wild-type activity after incubation in 30 mM H<sub>2</sub>O<sub>2</sub> by flow cytometry with fluorescein-tyramide as a substrate. To preserve the genotype-phenotype connection, the LiP mutants were displayed on the yeast cell surface. Two rounds of sorting were performed, recovered colonies were then screened in microtiter plates, and activity analysis revealed a significant increase in the percentage of cells expressing LiP variants with higher oxidative stability than wtLiP. Two rounds of sorting increased the proportion of more-stable variants from 1.4% in the original library to 52.3%. The most stable variants after two rounds of sorting featured between two and four mutations and retained up to 80% of initial activity after 1 h incubation in 30 mM H<sub>2</sub>O<sub>2</sub>. We for the first-time applied flow cytometry for screening of any ligninolytic peroxidase library. Obtained results suggest that developed system may be applied for improvement of industrially important characteristics of lignin peroxidase.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">32035791</PMID><DateCompleted><Year>2020</Year><Month>08</Month><Day>10</Day></DateCompleted><DateRevised><Year>2020</Year><Month>08</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1347-4421</ISSN><JournalIssue CitedMedium="Internet"><Volume>129</Volume><Issue>6</Issue><PubDate><Year>2020</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal of bioscience and bioengineering</Title><ISOAbbreviation>J Biosci Bioeng</ISOAbbreviation></Journal><ArticleTitle>Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability.</ArticleTitle><Pagination><MedlinePgn>664-671</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1389-1723(19)31033-3</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jbiosc.2019.12.009</ELocationID><Abstract><AbstractText>Lignin peroxidase (LiP) is a heme-containing oxidoreductase that oxidizes structurally diverse substrates in an H<sub>2</sub>O<sub>2</sub>-dependent manner. Its ability to oxidize many pollutants makes it suitable for bioremediation applications and an ideal candidate for optimization by mutagenesis and selection. In order to increase oxidative stability of LiP we generated a random mutagenesis library comprising 10<sup>6</sup> mutated LiP genes and screened for expressed enzymes with higher than wild-type activity after incubation in 30 mM H<sub>2</sub>O<sub>2</sub> by flow cytometry with fluorescein-tyramide as a substrate. To preserve the genotype-phenotype connection, the LiP mutants were displayed on the yeast cell surface. Two rounds of sorting were performed, recovered colonies were then screened in microtiter plates, and activity analysis revealed a significant increase in the percentage of cells expressing LiP variants with higher oxidative stability than wtLiP. Two rounds of sorting increased the proportion of more-stable variants from 1.4% in the original library to 52.3%. The most stable variants after two rounds of sorting featured between two and four mutations and retained up to 80% of initial activity after 1 h incubation in 30 mM H<sub>2</sub>O<sub>2</sub>. We for the first-time applied flow cytometry for screening of any ligninolytic peroxidase library. Obtained results suggest that developed system may be applied for improvement of industrially important characteristics of lignin peroxidase.</AbstractText><CopyrightInformation>Copyright © 2020 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ilić Đurđić</LastName><ForeName>Karla</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade, Serbia. Electronic address: karlailic@chem.bg.ac.rs.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ece</LastName><ForeName>Selin</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>PerkinElmer Chemagen Technologie GmbH, Arnold-Sommerfeld-Ring 2, 52499 Baesweiler, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ostafe</LastName><ForeName>Raluca</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Purdue Institute of Inflammation, Immunology and Infectious Disease, Molecular Evolution, Protein Engineering and Production, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907, USA; Institute of Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vogel</LastName><ForeName>Simon</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Balaž</LastName><ForeName>Ana Marija</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Institute of Chemistry Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000 Belgrade, Serbia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schillberg</LastName><ForeName>Stefan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fischer</LastName><ForeName>Rainer</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Institute of Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany; Departments of Biological Sciences and Chemistry, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Prodanović</LastName><ForeName>Radivoje</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade, Serbia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>02</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>J Biosci Bioeng</MedlineTA><NlmUniqueID>100888800</NlmUniqueID><ISSNLinking>1347-4421</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="C042858">lignin peroxidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005434" MajorTopicYN="N">Flow Cytometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="N">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Chimera</Keyword><Keyword MajorTopicYN="N">Directed evolution</Keyword><Keyword MajorTopicYN="N">Fluorescence activated cell sorting</Keyword><Keyword MajorTopicYN="N">Hydrogen-peroxide stability</Keyword><Keyword MajorTopicYN="N">Yeast surface display</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>10</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>12</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>12</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>2</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>2</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32035791</ArticleId><ArticleId IdType="pii">S1389-1723(19)31033-3</ArticleId><ArticleId IdType="doi">10.1016/j.jbiosc.2019.12.009</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Serbie</li><li>États-Unis</li></country><region><li>District de Cologne</li><li>Indiana</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Aix-la-Chapelle</li></settlement></list><tree><country name="Serbie"><noRegion><name sortKey="Ili Ur I, Karla" sort="Ili Ur I, Karla" uniqKey="Ili Ur I K" first="Karla" last="Ili Ur I">Karla Ili Ur I</name></noRegion><name sortKey="Balaz, Ana Marija" sort="Balaz, Ana Marija" uniqKey="Balaz A" first="Ana Marija" last="Balaž">Ana Marija Balaž</name><name sortKey="Prodanovi, Radivoje" sort="Prodanovi, Radivoje" uniqKey="Prodanovi R" first="Radivoje" last="Prodanovi">Radivoje Prodanovi</name></country><country name="Allemagne"><noRegion><name sortKey="Ece, Selin" sort="Ece, Selin" uniqKey="Ece S" first="Selin" last="Ece">Selin Ece</name></noRegion><name sortKey="Ostafe, Raluca" sort="Ostafe, Raluca" uniqKey="Ostafe R" first="Raluca" last="Ostafe">Raluca Ostafe</name><name sortKey="Schillberg, Stefan" sort="Schillberg, Stefan" uniqKey="Schillberg S" first="Stefan" last="Schillberg">Stefan Schillberg</name><name sortKey="Vogel, Simon" sort="Vogel, Simon" uniqKey="Vogel S" first="Simon" last="Vogel">Simon Vogel</name></country><country name="États-Unis"><region name="Indiana"><name sortKey="Fischer, Rainer" sort="Fischer, Rainer" uniqKey="Fischer R" first="Rainer" last="Fischer">Rainer Fischer</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000025 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000025 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhanerochaeteV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32035791
|texte= Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32035791" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhanerochaeteV1
| This area was generated with Dilib version V0.6.37. |  |